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Subhashini Pothukuchi has Published 45 Articles

Subhashini Pothukuchi
2K+ Views
Introduction Composting is the process of breaking down organic waste materials into nutrient-rich soil amendments that can be used to enrich gardens, farms, and lawns. This process is achieved through the natural decomposition of organic matter by microorganisms such as bacteria, fungi, and other decomposers. Composting is an environmentally friendly ... Read More

Subhashini Pothukuchi
1K+ Views
Introduction Plant tissue culture is a valuable tool in plant biotechnology. It involves the manipulation of plant cells, tissues, and organs in vitro under sterile conditions. The technique has various applications in plant breeding, genetic engineering, and plant propagation. Two techniques commonly used in plant tissue culture are anther culture ... Read More

Subhashini Pothukuchi
592 Views
Introduction Amniotic fluid is a clear, slightly yellowish liquid that surrounds the developing fetus in the uterus during pregnancy. It plays a crucial role in fetal development and protection, and any abnormalities in its production or composition can have significant consequences for the developing fetus. The amniotic fluid is produced ... Read More

Subhashini Pothukuchi
851 Views
IntroductionStem-loops or hairpin loops are essential structural elements found in nucleic acids, specifically in RNA molecules. They are formed by base pairing between complementary nucleotides within the same strand, leading to the formation of a double-stranded stem and a looped region. Stem-loops have various biological functions, including gene expression regulation, ... Read More

Subhashini Pothukuchi
366 Views
Introduction RNA pseudoknots are secondary structures that have a crucial role in regulating gene expression and protein synthesis in all organisms. These structures are formed when a single-stranded RNA molecule folds back on itself, creating a stem-loop structure that is interrupted by an additional stem-loop structure. The result is a ... Read More

Subhashini Pothukuchi
636 Views
Introduction A mutagen is any substance or agent that causes a genetic mutation. Mutations refer to changes in the DNA sequence of an organism's genetic material, which can occur naturally or because of exposure to mutagenic agents. Mutations are a fundamental process in evolution, and can either be beneficial, ... Read More

Subhashini Pothukuchi
394 Views
Introduction Deoxyribonucleic acid, commonly known as DNA, is the fundamental molecule that contains genetic information in all living organisms. It is a long chain-like structure composed of four nucleotides – adenine, cytosine, guanine, and thymine – that are arranged in a specific sequence. DNA sequences are not random, but they ... Read More

Subhashini Pothukuchi
705 Views
Introduction Fosmids are vectors used for cloning large fragments of DNA. They were developed in the 1990s to improve on the capabilities of other cloning vectors, such as bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). Fosmids are particularly useful for studying large and complex genomes, and they have ... Read More

Subhashini Pothukuchi
724 Views
Introduction Exonucleases are enzymes that cleave nucleotides from the end of DNA or RNA molecules. These enzymes are important for a wide variety of biological processes, including DNA replication, DNA repair, and RNA processing. In this article, we will discuss the properties of exonucleases and their applications in biotechnology and ... Read More

Subhashini Pothukuchi
388 Views
Introduction Endonucleases are a class of enzymes that can cleave the phosphodiester bonds within a DNA or RNA molecule. These enzymes play a crucial role in various biological processes such as DNA repair, replication, transcription, and recombination. Endonucleases are also widely used in biotechnology, particularly in molecular biology, for various ... Read More