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Subhashini Pothukuchi has Published 45 Articles
Subhashini Pothukuchi
585 Views
IntroductionMutagenesis refers to the process of inducing mutations in the genetic material of an organism. Mutations are changes that occur in the DNA sequence, either naturally or through the use of mutagens. These changes can lead to alterations in the phenotype of an organism, which may be beneficial, detrimental or ... Read More
Subhashini Pothukuchi
3K+ Views
Introduction Cosmid vectors are plasmid vectors that are used to carry large fragments of DNA (approximately 35-45kb) in molecular biology research. These vectors are derived from the DNA of a bacteriophage known as P1, which can integrate its DNA into the bacterial genome. Cosmid vectors are widely used in the ... Read More
Subhashini Pothukuchi
47 Views
Introduction The genetic code is the set of instructions that specifies the amino acid sequence of a protein. In bacteria, the genetic code is carried by the messenger RNA (mRNA) which is translated by the ribosome. Transfer RNAs (tRNAs) play a crucial role in this process by bringing the correct ... Read More
Subhashini Pothukuchi
132 Views
Introduction Signature-tagged mutagenesis (STM) is a powerful genetic technique used in molecular biology to identify genes that are essential for the survival or virulence of a pathogen. STM is a useful tool for studying bacterial pathogens because it allows researchers to identify genes that are required for the pathogen to ... Read More
Subhashini Pothukuchi
595 Views
Introduction DNA, or deoxyribonucleic acid, is the genetic material present in all living organisms. It contains the instructions necessary for the development and function of living organisms. Within the vast genomic landscape, one type of DNA stands out for its repetitive and non-coding nature - Satellite DNA. Satellite DNA ... Read More
Subhashini Pothukuchi
107 Views
Introduction Riboswitches and the RNA world are two fascinating topics in the field of molecular biology. Riboswitches are RNA molecules that can change their conformation in response to the binding of a specific small molecule. RNA world, on the other hand, is a hypothesis that suggests that life on Earth ... Read More
Subhashini Pothukuchi
109 Views
Introduction Programmed cell death (PCD) is a crucial process in the life cycle of cells. It is a genetically controlled and regulated process that leads to the death of cells in a controlled manner. PCD is an essential process for maintaining normal cell growth and development in multicellular organisms, and ... Read More
Subhashini Pothukuchi
563 Views
Introduction Primer walking is a technique used in molecular biology for DNA sequencing. It involves designing a series of overlapping primers to amplify small regions of DNA, one at a time, until the entire sequence of interest has been covered. The resulting sequence data can be used to study the ... Read More
Subhashini Pothukuchi
2K+ Views
Introduction The pBR322 vector is a plasmid vector that has been extensively used in genetic engineering and molecular biology research for over four decades. It was one of the first plasmids to be commercially available and has since become a widely used tool in recombinant DNA technology. In this article, ... Read More
Subhashini Pothukuchi
433 Views
Introduction A palindromic sequence is a sequence of characters that remains the same when read forwards and backwards. In other words, a palindromic sequence reads the same way in both directions. Palindromes are fascinating from a linguistic perspective and are used in various applications, including genetics, linguistics, and cryptography. ... Read More
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