Found 36 Articles for Medical Biotechnology

Introduction Cosmid vectors are plasmid vectors that are used to carry large fragments of DNA (approximately 35-45kb) in molecular biology research. These vectors are derived from the DNA of a bacteriophage known as P1, which can integrate its DNA into the bacterial genome. Cosmid vectors are widely used in the field of molecular genetics for the construction of genomic libraries, the identification and cloning of genes, and the production of large amounts of recombinant DNA. Cosmid vectors were first developed in the 1970s to clone large fragments of DNA. At the time, researchers were using bacteriophages to clone DNA, ... Read More

Introduction The genetic code is the set of instructions that specifies the amino acid sequence of a protein. In bacteria, the genetic code is carried by the messenger RNA (mRNA) which is translated by the ribosome. Transfer RNAs (tRNAs) play a crucial role in this process by bringing the correct amino acid to the ribosome, according to the codon on the mRNA. Each tRNA is specific for a particular amino acid and has an anticodon that pairs with the codon on the mRNA. In this article, we will focus on the synthesis of the gene for bacterial tyrosine ... Read More

Introduction Signature-tagged mutagenesis (STM) is a powerful genetic technique used in molecular biology to identify genes that are essential for the survival or virulence of a pathogen. STM is a useful tool for studying bacterial pathogens because it allows researchers to identify genes that are required for the pathogen to survive and cause disease. STM is a method that involves the random mutagenesis of a pathogen's genome, followed by the identification of mutants that are unable to survive or grow under specific conditions. STM was first developed in the 1980s by Stanley Falkow and his colleagues as a tool ... Read More

Introduction DNA, or deoxyribonucleic acid, is the genetic material present in all living organisms. It contains the instructions necessary for the development and function of living organisms. Within the vast genomic landscape, one type of DNA stands out for its repetitive and non-coding nature - Satellite DNA. Satellite DNA It is a unique type of DNA that is characterized by its highly repetitive nature. These sequences of DNA do not code for proteins but have crucial roles in genome structure, function, and evolution. In this article, we will explore the origin, structure, and types of Satellite DNA. Origin ... Read More

Introduction Riboswitches and the RNA world are two fascinating topics in the field of molecular biology. Riboswitches are RNA molecules that can change their conformation in response to the binding of a specific small molecule. RNA world, on the other hand, is a hypothesis that suggests that life on Earth might have originated from RNA molecules, which acted as both genetic material and enzymes. In this article, we will discuss these two topics in detail, their significance in biology, and their potential applications. Riboswitches Riboswitches are regulatory elements present in the untranslated regions (UTRs) of certain messenger RNAs (mRNAs). These ... Read More

Introduction Programmed cell death (PCD) is a crucial process in the life cycle of cells. It is a genetically controlled and regulated process that leads to the death of cells in a controlled manner. PCD is an essential process for maintaining normal cell growth and development in multicellular organisms, and it plays a crucial role in various physiological processes, including tissue remodelling, immune system regulation, and elimination of abnormal cells. The process of PCD is mediated by a series of molecular events, which are regulated by a complex network of signalling pathways. These pathways are activated by various internal and ... Read More

Introduction Primer walking is a technique used in molecular biology for DNA sequencing. It involves designing a series of overlapping primers to amplify small regions of DNA, one at a time, until the entire sequence of interest has been covered. The resulting sequence data can be used to study the structure and function of genes, identify mutations, and diagnose genetic disorders. The concept of primer walking was first proposed in the early 1990s, when automated DNA sequencing machines were becoming more widely available. Prior to this, DNA sequencing was a labor-intensive process that involved manually sequencing short pieces of DNA ... Read More

Introduction The pBR322 vector is a plasmid vector that has been extensively used in genetic engineering and molecular biology research for over four decades. It was one of the first plasmids to be commercially available and has since become a widely used tool in recombinant DNA technology. In this article, we will explore the structure, features, and applications of the pBR322 vector. Definition The pBR322 vector is a circular double-stranded DNA molecule that is approximately 4361 base pairs long. It is an E. coli plasmid that was first constructed in 1977 by Bolivar and Rodriguez, and it is named ... Read More

Introduction A palindromic sequence is a sequence of characters that remains the same when read forwards and backwards. In other words, a palindromic sequence reads the same way in both directions. Palindromes are fascinating from a linguistic perspective and are used in various applications, including genetics, linguistics, and cryptography. This article represents the following topic like definition, examples, and importance of palindromic sequences. Definition A palindrome is a word, phrase, or sequence of characters that is read the same way in both directions. A palindromic sequence, therefore, is a sequence of characters that has this property. Palindromic sequences ... Read More

Introduction Open Reading Frames (ORFs) are a fundamental concept in molecular biology, and they play a crucial role in understanding the structure and function of genes. ORFs are sequences of DNA that are transcribed into RNA and translated into proteins. They are characterized by a start codon (usually AUG) and a stop codon (UAA, UAG, or UGA) that mark the beginning and end of the coding region. ORFs are typically identified by computer programs that analyze the genome sequence and predict the locations of coding regions. These programs use a variety of algorithms to identify potential ORFs, such as ... Read More