Found 292 Articles for Biotechnology

Introduction Nutrient media is a type of growth medium that is commonly used in microbiology to culture and grow various microorganisms. It is a complex mixture of nutrients that are necessary for the growth of microorganisms such as bacteria, yeast, and fungi. It can be made using a variety of different ingredients, each of which serves a specific purpose in promoting the growth of microorganisms. Discussed below are different types of nutrient media and their preparation. Types of Nutrient Media There are many different types of nutrient media that are used in microbiology, each of which is designed to ... Read More

An Overview Polyacrylamide gel electrophoresis, or PAGE, is a powerful tool used by scientists to separate and analyse proteins, DNA, and RNA. This technique is widely used in various fields, including biochemistry, molecular biology, genetics, and proteomics, to study the properties of macromolecules and their interactions. Polyacrylamide Gel Electrophoresis with 15-Lane Blue Loading Buffer Given below is the purpose of polyacrylamide gel electrophoresis, its history, and how it works. We will also elucidate the different types of PAGE and their applications, as well as the advantages and limitations of this technique. History of Polyacrylamide Gel Electrophoresis The development of ... Read More

What is Synthetic Seed Technology? Synthetic seed technology is a plant biotechnology technique that involves the production of artificial seeds from plant embryos. The process of producing synthetic seeds involves encapsulating a somatic embryo or shoot-tip culture in a protective coating. The protective coating is composed of a mixture of gelling agents, nutrients, and growth regulators that promote the growth and development of the plant embryo. Synthetic seeds offer several advantages over conventional seeds. They are easier to handle, transport, and store. They can also be used for planting in areas with adverse environmental conditions, such as drought-prone regions ... Read More

Introduction Gel electrophoresis is a widely used analytical technique in molecular biology and biochemistry that allows the separation of DNA, RNA, and protein molecules based on their size, charge, and other physical properties. It is a simple and effective way to isolate and purify specific molecules from complex mixtures, such as DNA fragments, RNA transcripts, or proteins. Gel electrophoresis is used in a variety of applications, including genetic research, medical diagnostics, and biotechnology. Herewith is a comprehensive overview of gel electrophoresis, including its definition, principle, steps, and uses. Definition of Gel Electrophoresis Gel electrophoresis is a laboratory technique used ... Read More

Introduction Affinity chromatography is a powerful technique used in the field of biochemistry to separate and purify proteins, enzymes, and other biomolecules from a complex mixture of biological samples. This technique relies on the specific interaction between a target molecule and a ligand that is immobilized on a chromatographic support. Affinity chromatography is widely used in the purification of biological molecules, and its success can be attributed to its high specificity, high yield, and ease of use. Affinity chromatography used in isolation of biological molecules Given below is an overview of the principles, techniques, and applications of affinity ... Read More

An Insight Bacterial growth curve is a graphical representation of the growth rate of bacteria in a closed environment. It provides us with a valuable insight into the life cycle of bacteria and their behaviour under different environmental conditions. A typical bacterial growth curve consists of four phases − Lag phase. Exponential phase. Stationary phase. Death phase. Topic herewith discusses the phases in detail. Lag Phase The lag phase is the first phase of the bacterial growth curve. In this phase, bacteria are adjusting to their new environment and preparing for growth. During this phase, there is ... Read More

Introduction Molecular biology is the study of the molecular basis of biological activity. It encompasses the structure and function of macromolecules such as DNA, RNA, and proteins, as well as the interactions between these molecules. The techniques used in molecular biology have revolutionized the field of genetics and have enabled researchers to study the molecular mechanisms underlying a wide range of biological processes. Discussed below are the basic techniques used in molecular biology and how they are used to study the structure, function, and regulation of biological molecules. Polymerase Chain Reaction (PCR) Polymerase chain reaction (PCR) is a technique ... Read More

Introduction Symmetric hybrids are chromosomes that have a balanced distribution of genetic information between two homologous chromosomes. Homologous chromosomes are a pair of chromosomes that carry the same genes, but they may carry different alleles (variations) of those genes. In symmetric hybrids, the genetic information on both chromosomes is equally shared between them. This means that the chromosomes are identical in terms of their genetic content. Asymmetric hybrids, on the other hand, are chromosomes that have an unbalanced distribution of genetic information between two homologous chromosomes. In this case, one of the chromosomes has more genetic material than the ... Read More

Introduction Genetic transformation is an essential tool in the field of plant biotechnology that allows the introduction of desired traits into plants. Plant transformation vectors are genetic tools that enable the transfer of foreign genes into plant cells. These vectors act as vehicles to carry the desired genes into the plant genome, where they can be stably integrated and expressed. Below we shall investigate an overview of plant transformation vectors, their components, and their use in plant biotechnology. Plant Transformation Vectors-Their Components Plant transformation vectors are circular DNA molecules that can be introduced into plant cells using various techniques ... Read More

Introduction Good Laboratory Practice (GLP) is a set of guidelines, procedures, and standards for conducting non-clinical laboratory studies in accordance with regulatory requirements. GLP regulations are put in place to ensure the accuracy, reliability, and integrity of data generated from laboratory studies. In this article, we will discuss what GLP is, why it is important, and the key elements of good laboratory practices. What are Good Laboratory Practices (GLP)? GLP refers to a set of principles that are designed to ensure the quality and reliability of laboratory studies. These principles encompass all aspects of laboratory work, including the design, ... Read More