Found 3 Articles for Chromatography

Introduction Affinity chromatography is a powerful technique used in the field of biochemistry to separate and purify proteins, enzymes, and other biomolecules from a complex mixture of biological samples. This technique relies on the specific interaction between a target molecule and a ligand that is immobilized on a chromatographic support. Affinity chromatography is widely used in the purification of biological molecules, and its success can be attributed to its high specificity, high yield, and ease of use. Affinity chromatography used in isolation of biological molecules Given below is an overview of the principles, techniques, and applications of affinity ... Read More

Introduction Ion exchange chromatography is a powerful technique for separating and purifying proteins, peptides, nucleic acids, and other charged biomolecules based on their net charge. The technique involves the use of a stationary phase consisting of a resin with fixed charged groups that bind to the target molecules based on their charge characteristics. The principle of ion exchange chromatography is based on the differential affinity of biomolecules for the charged groups on the resin, which enables selective separation of the target molecule from a complex mixture of biomolecules. The content below elaborates the principle, steps, and uses of ion ... Read More

Introduction Column chromatography is one of the most versatile and widely used techniques for separating and purifying mixtures of organic compounds. It is a type of liquid chromatography where the stationary phase is a solid adsorbent material packed into a column, and the mobile phase is a liquid or gas that flows through the column. This technique is based on the principles of adsorption, partition, and ion exchange, and it can be used to separate a wide range of compounds, including small molecules, proteins, and nucleic acids. In this article, we will discuss the principle, types, and significance of ... Read More