Found 4 Articles for Molecular Biology Techniques

An Overview Polyacrylamide gel electrophoresis, or PAGE, is a powerful tool used by scientists to separate and analyse proteins, DNA, and RNA. This technique is widely used in various fields, including biochemistry, molecular biology, genetics, and proteomics, to study the properties of macromolecules and their interactions. Polyacrylamide Gel Electrophoresis with 15-Lane Blue Loading Buffer Given below is the purpose of polyacrylamide gel electrophoresis, its history, and how it works. We will also elucidate the different types of PAGE and their applications, as well as the advantages and limitations of this technique. History of Polyacrylamide Gel Electrophoresis The development of ... Read More

Introduction Gel electrophoresis is a widely used analytical technique in molecular biology and biochemistry that allows the separation of DNA, RNA, and protein molecules based on their size, charge, and other physical properties. It is a simple and effective way to isolate and purify specific molecules from complex mixtures, such as DNA fragments, RNA transcripts, or proteins. Gel electrophoresis is used in a variety of applications, including genetic research, medical diagnostics, and biotechnology. Herewith is a comprehensive overview of gel electrophoresis, including its definition, principle, steps, and uses. Definition of Gel Electrophoresis Gel electrophoresis is a laboratory technique used ... Read More

Introduction Affinity chromatography is a powerful technique used in the field of biochemistry to separate and purify proteins, enzymes, and other biomolecules from a complex mixture of biological samples. This technique relies on the specific interaction between a target molecule and a ligand that is immobilized on a chromatographic support. Affinity chromatography is widely used in the purification of biological molecules, and its success can be attributed to its high specificity, high yield, and ease of use. Affinity chromatography used in isolation of biological molecules Given below is an overview of the principles, techniques, and applications of affinity ... Read More

Introduction Agarose gel electrophoresis is a laboratory technique used to separate DNA, RNA, or proteins based on their size and charge. It is an essential method in molecular biology, biochemistry, and genetics research, and is widely used for the analysis of nucleic acids in forensic, medical, and biotechnological applications. Definition of Agarose Gel Electrophoresis Agarose gel electrophoresis is a technique that involves the separation of nucleic acids or proteins in a gel matrix made of agarose. The matrix is placed in an electric field, and the charged molecules migrate through the matrix based on their size, shape, and charge. ... Read More