Found 4 Articles for Molecular Biology

Introduction The operon concept is a fundamental concept in molecular biology that explains the regulation of gene expression in prokaryotes. The operon concept was first proposed by Francois Jacob and Jacques Monod in 1961, based on their studies of the lac operon in E. coli bacteria. The operon concept describes a group of genes that are transcribed together as a single unit, which is controlled by a regulatory region known as the operator. In this article, we will delve into the structure and functions of the operon concept, as well as some unknown facts. What is the Operon Concept? ... Read More

An Insight Bacterial growth curve is a graphical representation of the growth rate of bacteria in a closed environment. It provides us with a valuable insight into the life cycle of bacteria and their behaviour under different environmental conditions. A typical bacterial growth curve consists of four phases − Lag phase. Exponential phase. Stationary phase. Death phase. Topic herewith discusses the phases in detail. Lag Phase The lag phase is the first phase of the bacterial growth curve. In this phase, bacteria are adjusting to their new environment and preparing for growth. During this phase, there is ... Read More

Introduction Molecular biology is the study of the molecular basis of biological activity. It encompasses the structure and function of macromolecules such as DNA, RNA, and proteins, as well as the interactions between these molecules. The techniques used in molecular biology have revolutionized the field of genetics and have enabled researchers to study the molecular mechanisms underlying a wide range of biological processes. Discussed below are the basic techniques used in molecular biology and how they are used to study the structure, function, and regulation of biological molecules. Polymerase Chain Reaction (PCR) Polymerase chain reaction (PCR) is a technique ... Read More

Introduction Plasmids are small, circular DNA molecules that are separate from the bacterial chromosome. They are found in bacteria and are capable of self-replication. Plasmids often carry genes that confer some selective advantage to the bacterium, such as antibiotic resistance. As such, they are important tools in genetic engineering and biotechnology. In this article, we will discuss the process of isolating plasmid DNA from bacteria. Isolation of Plasmid DNA from Bacteria The process of isolating plasmid DNA from bacteria involves several steps, which are outlined below. Step 1 Bacterial Culture: The first step in isolating plasmid DNA from bacteria ... Read More