Found 292 Articles for Biotechnology

Introduction Protoplast culture is a laboratory technique that involves the isolation of the plant cell from the cell wall. The protoplasts can then be cultured in vitro, allowing for the study of cellular processes, including cell division, differentiation, and regeneration. Protoplast The technique has many applications in plant biotechnology, including the production of new crop varieties, genetic engineering, and the production of plant secondary metabolites. Below we shall look at the importance of protoplast culture in plant regeneration and how it is used to produce new plants. What Are Protoplasts? Protoplasts are cells that have had their cell walls ... Read More

Introduction Handling and sterilization of plant material is an important aspect of any laboratory or plant research facility. Plant material, such as leaves, stems, and seeds, can be contaminated with microbes and other pathogens that can affect the results of research. To ensure the accuracy and reliability of research, it is important to properly handle and sterilize the plant material. In this tutorial, we will discuss various methods of handling and sterilization of plant material. Part 1: Handling of Plant Material Handling of plant material involves the proper collection and preparation of the sample for sterilization. Before collecting plant ... Read More

Introduction Bacteriophages, or phage’s, are viruses that infect and replicate inside bacteria. They are widespread in nature and are found in almost every environment, including soil, water, and the human body. Phage’s have been used for over a century as a tool to study bacteria, and they have also been explored as potential alternatives to antibiotics for treating bacterial infections. In this tutorial, we will discuss the growth of Lambda phage and the isolation of phage DNA. Growth of Lambda Phage Lambda phage is a temperate phage that infects Escherichia coli (E. coli) bacteria. It has a linear, double-stranded ... Read More

Introduction Transgenic organisms are living organisms whose genetic material has been artificially modified to introduce specific traits or characteristics. This can be done through genetic engineering, which involves the insertion of specific genes or DNA sequences into an organism's genome. Transgenic organisms have become increasingly important in various fields, including agriculture, medicine, and biotechnology. Genetic and molecular analyses are essential in understanding the mechanisms underlying transgenesis and in evaluating the potential risks and benefits associated with the use of transgenic organisms. Genetic Analysis of Transgenics Genetic analysis involves the study of an organism's genetic material, including its DNA, ... Read More

Embryo Rescue -A Milestone Embryo rescue is a technique that involves the rescue of developing embryos from immature or unpollinated ovules of sexually incompatible plant crosses. It enables the development of hybrid embryos that would otherwise not survive to maturity. Embryo rescue was first introduced in the early 20th century and has since been extensively used in plant breeding, particularly in generating interspecific and intergeneric hybrids. The basis of embryo rescue is the manipulation of the in vitro culture conditions to provide the developing embryo with the necessary nutrients and support for its growth and development. The success of ... Read More

Cyborgs - An Insight With the increasing use of technology and artificial intelligence, the concept of Cyborgs has gained significant attention. Cyborgs, also known as Cyborg Hybrids, are a combination of biological and technological components. The term 'cyborg' refers to an organism that has both organic and synthetic parts, while 'hybrid' refers to a combination of two different things. Cyborgs are an exciting field of study and have many potential applications, but they also come with their own set of limitations. Below we have discussed about the applications and limitations of Cyborgs and the ethical implications of this emerging ... Read More

Introduction Column chromatography is one of the most versatile and widely used techniques for separating and purifying mixtures of organic compounds. It is a type of liquid chromatography where the stationary phase is a solid adsorbent material packed into a column, and the mobile phase is a liquid or gas that flows through the column. This technique is based on the principles of adsorption, partition, and ion exchange, and it can be used to separate a wide range of compounds, including small molecules, proteins, and nucleic acids. In this article, we will discuss the principle, types, and significance of ... Read More

Introduction Chromatography is a separation technique that is used to separate and identify individual components of a mixture. The technique relies on the differential affinities of the components of the mixture for a stationary phase and a mobile phase The stationary phase is a material that is fixed in place and the mobile phase is a fluid that moves through the stationary phase. The components of the mixture interact differently with the stationary phase and the mobile phase, which results in their separation. Chromatography has a wide range of applications in various fields, including chemistry, biochemistry, pharmaceuticals, food science, ... Read More

Introduction Centrifugal force is a term used to describe the apparent force that acts on an object moving in a circular path. It is often referred to as a "fictitious" force because it does not have a physical origin, but rather is a result of the object's motion. Despite its lack of a physical origin, the centrifugal force plays a crucial role in many aspects of our lives, from amusement park rides to the operation of centrifuges in laboratories. In this article, we will explore the principle behind centrifugal force, the formula used to calculate it, and its applications in ... Read More

Introduction Agarose gel electrophoresis is a laboratory technique used to separate DNA, RNA, or proteins based on their size and charge. It is an essential method in molecular biology, biochemistry, and genetics research, and is widely used for the analysis of nucleic acids in forensic, medical, and biotechnological applications. Definition of Agarose Gel Electrophoresis Agarose gel electrophoresis is a technique that involves the separation of nucleic acids or proteins in a gel matrix made of agarose. The matrix is placed in an electric field, and the charged molecules migrate through the matrix based on their size, shape, and charge. ... Read More