Found 12 Articles for Analytical Chemistry

Introduction Principle of Spectroscopy is how the spectra can absorb UV light. The absorption of light refers to a vital chapter in chemistry. Absorption of ultraviolet radiation results in an excitation of the electrons available in the substances. About Spectrophotometer A spectrophotometer refers to a scientific instrument is primarily utilised for the analysis of electromagnetic radiation from the light to the sample and about its absorption wavelength. The absorption is measurement using the spectral components. The spectrophotometer is used to measure molecular spectroscopy. In physics, radiation is the emission or transmission of energy in the form of wavelength or particles. ... Read More

Introduction Chromatography is a laboratory technique, especially in the analytical field used to separate a mixture of components present in a chemical species. There are different types of chromatographic techniques For the separation of mixtures into their units in the laboratory, the chromatographic technique is used. So, the method which involves the separation of a specific mixture into its respective elements is given the name chromatography. It is done by using a mobile phase and stationary phase. The mobile phase carries away distinct components through the stationary phase present in a mixture because the effect of the solvent is different ... Read More

Introduction: What is an Endpoint? It is the stage of a titration at which the colour changes. Acids, as well as bases, are normally colourless liquids. As a result, an indicator that may alter the colour of the reaction medium with variations in pH is employed to indicate the end of the neutralisation reaction of an acid with a base. Phenolphthalein is a typical indicator used in acid-base processes. This indicator's colour changes between 8.3 (colourless) to 10.0. (pink). However, this indicator is ineffective for reactions occurring at low pH levels. As a result, the indication should be picked carefully. ... Read More

Introduction The technique of separating a mixture of chemicals into their constituents based on their interactions with an inert matrix is known as chromatography. However, it is much more than a basic procedure; it is an essential aspect of research that encompasses bio and physical chemistry, chemical engineering, or other domains. The IUPAC definition of chromatography is "the separation of sample components following their dispersion between two phases." It is an important biophysical technique for separating, identifying, and then purifying the components of a mixture for identification but also quantification. There are several types of chromatography, which differ in the ... Read More

Introduction Conductometric titration is one of the types of titrations where chemical reactions are carried out with the help of a conductometer. Like in any other titration, an indicator is used to determine the endpoint; here a conductometer is used to find the endpoint of a reaction. It works on the principle of replacing one ion with another in the reaction mixture. This exchange of ions within the solution helps measure the changes in conductance. These changes help in reaching the endpoint, which tells a reaction is completed. Such a type of titration in which the endpoint is determined ... Read More

Introduction M. S. Tswett, a Russian scientist, introduced the chromatographic technique. The technique that has been used for the separation of particles present in a given mixture is chromatography. In which the mobile phase moves through the stationary phase and the separation of particles present in the mixture takes place according to the affinity with the solvent used. There are distinct chromatographic techniques and are based on the different mobile and stationary phases. Column chromatography is one such chromatographic technique. The stationary phase is a column of adsorbent coated with the mixture and the mobile phase is a solvent. The ... Read More

Introduction Titration or titrimetry is an analytical method that is used for the detection of an unknown amount of concentration of the unknown analyte. It is a volume measurement so it is also called a volumetric method. A standard solution of a reagent or titrant is prepared and it will then react with the analyte to obtain its concentration. The volume in which the reagent or titrant reacted is called the titrant volume. There are various titrimetry methods. Complexometric titration is one among them. It is a titration in which the formation of a colored coordination complex is indicated as ... Read More

Introduction Titration is a quantitative analysis used in chemistry used to identify the strength or concentration of a given unknown chemical solution by analysing it with a known solution concentration with the presence of an indicator. Both oxidation and reduction happen at the same time. Titration is a process for calculating the quantity of one unknown solution concentration by other solutions. An Indicator is required to detect the endpoint of equivalence. It generally occurs with colour change. What is Titration? Chemical reaction occurs with the interchange of ions when certain chemical species are in contact with each other. Titration ... Read More

Introduction Centrifuge and its application explained by Sir Isaac Newton in his second law of motion. He outlined that a moving body when it is free, travels in a straight line. The opposite in his theory is the restraining force when it changes its position. This force generally exerts pressure against the restraining force so that it can travel in a straight line. The theory about it is that the force extends by extending the angular velocity of rotation and the mass of the object. The centrifugal force sometimes connected straight with the pull of gravity of the object. ... Read More

Introduction The mass spectrometers are mainly utilized in order to detect several unknown components through molecular weight and it also helps in the determination of phenomenon. This process also helps to quantify the already detected components and determine the structure and chemical properties of the molecules that are present in the given sample. The mass spectrometer generally performs through three major steps including Ionization Source, Ion Detection System, and Mass Analyzer. The two instruments that are utilized in various technical fields to study mass spectrometry is also determined as mass spectroscopy, are mass spectrometers and mass spectrographs. What is Mass ... Read More