Found 1 Articles for Lab Techniques

Introduction Ion exchange chromatography is a powerful technique for separating and purifying proteins, peptides, nucleic acids, and other charged biomolecules based on their net charge. The technique involves the use of a stationary phase consisting of a resin with fixed charged groups that bind to the target molecules based on their charge characteristics. The principle of ion exchange chromatography is based on the differential affinity of biomolecules for the charged groups on the resin, which enables selective separation of the target molecule from a complex mixture of biomolecules. The content below elaborates the principle, steps, and uses of ion ... Read More