Validation & Verification of Analytical Methods
The simplest ways of measurement uncertainty calculation and evaluation of method performance parameters
Updated on Sep, 2023
Language - English
Research Methods,METHODS,Quality Management,ISO 9001,Business
Course Description
This training course is the easiest and most completed material for every analyst who needs to start in laboratory fields and for experience analysts too. not only theoretical but also practical training using excel sheets for calculation where it includes: Full validation example (excel sheet contains validation up to measurement uncertainty calculation for analysis and for sampling in a very easy way), control charts ready to be used for any method (accuracy and precision charts), ANOVA excel sheet ready to be used and also guidelines followed in this course attached with videos. After you will finalize this training and apply every step you will be able o validate or verify any method and that's very important to be accredited according to ISO 17025/2017 edition.
Note: All guidelines used are attached.
Learn with me how to develop methods for the analysis of the analyte of interest. What are the studies required to develop any method?
Matrix effect study, the chemistry of target analytes, and following steps to get the required method for analytes of interest.
How to select the instrument which is fit for the method.
Learn also how to evaluate most of the performance parameters according to international guidelines to meet the requirements of ISO 17025/2017 edition.
Learn the differences between verification & validation of methods and when you need to verify or validate the method.
Learn also how to ensure the validity of results and to get accurate and reliable results.
Learn how to calculate the limit of detection and the Limit of quantitation in 3 different ways.
Selectivity and specificity for analytes of interest.
Linearity in easy steps (Method linearity).
Learn the differences between Repeatability & reproducibility and how to ensure the repeatability of a specific method to analyze the required analytes.
Accuracy, precision & trueness.
Inter-laboratory comparison.
Intra-laboratory comparison.
Proficiency test (PT) and Certified reference materials (CRM).
Measurement uncertainty calculation for analytical measurement and for sampling, excel sheet attached.
Finally, create your own Excel sheet with a complete example in a very easy procedure.
Goals
What will you learn in this course:
Learn with me how to develop methods for the analysis of the analyte of interest. What are the studies required to develop any method?
Matrix effect study, the chemistry of target analytes, and following steps to get the required method for analytes of interest.
How to select the instrument which is fit for the method.
Learn also how to evaluate most of the performance parameters according to international guidelines to meet the requirements of ISO 17025/2017 edition.
Learn the differences between verification & validation of methods and when you need to verify or validate the method.
Learn also how to ensure the validity of results and to get accurate and reliable results.
Learn how to calculate the limit of detection and the Limit of quantitation in 3 different ways.
Selectivity and specificity for analytes of interest.
Linearity in easy steps (Method linearity).
Learn the differences between Repeatability & reproducibility and how to ensure the repeatability of a specific method to analyze the required analytes.
Accuracy, precision & trueness.
Inter-laboratory comparison.
Intra-laboratory comparison.
Proficiency test (PT) and Certified reference materials (CRM).
Measurement uncertainty calculation for analytical measurement and for sampling, excel sheet attached.
Finally, create your own Excel sheet with a complete example in a very easy procedure.
Prerequisites
What are the prerequisites for this course?
- A computer installed with EXCEL
- Internet connection
- Computer with any specification
- Listening to every lecture many times
- Basic computer fundamentals
- paper and pen
Curriculum
Check out the detailed breakdown of what’s inside the course
Method validation & verification
4 Lectures
-
Introduction to method validation
02:31
02:31
-
Method validation definition
05:04
05:04
-
Difference between method validation & method verification
12:09
12:09
-
Method selection & development
14:50
14:50
Method performance parameters as in EURACHEM guideline
6 Lectures
Measurement uncertainty calculation for analysis & sampling
4 Lectures
How to validate any method using excel sheet for calculation?
1 Lectures
Quality control charts
2 Lectures
Statistical tests
3 Lectures
Standard addition, external and internal standard
1 Lectures
Instructor Details
Mahmoud abdelkhabeer Mohamed
Mahmoud Abdelkhabeer, Head of veterinary drugs analysis in the central food laboratory in the ministry of health, Doha, Qatar I have been working in the field of laboratories for 18 years till now. During this time I have been involved in the development and validation of many methods such as Aflatoxin in nuts, spices, and cereals using HPLC Agilent, mycotoxins in cereals with LC-MS/MS Agilent 6470, and started a new section for analysis of veterinary drugs in food of animal origins such as sulfonamides, tetracyclines, hormones, amphenicol, and quinolones. These methods were developed and validated according to international guidelines and accredited to ISO 17025/2017 edition. I created a youtube channel that includes many lectures in our field and now I am trying to get more audiences to my work.
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