Techniques in Molecular Biology Articles

Growth of Lambda Phage and Isolation of Phage DNA

Anusha Karthik

Updated on 28-Mar-2023 363 Views

Introduction Bacteriophages, or phage’s, are viruses that infect and replicate inside bacteria. They are widespread in nature and are found in almost every environment, including soil, water, and the human body. Phage’s have been used for over a century as a tool to study bacteria, and they have also been explored as potential alternatives to antibiotics for treating bacterial infections. In this tutorial, we will discuss the growth of Lambda phage and the isolation of phage DNA. Growth of Lambda Phage Lambda phage is a temperate phage that infects Escherichia coli (E. coli) bacteria. It has a linear, double-stranded ...