Found 25 Articles for Genetic Transformation

Introduction Somatic hybridization is a recent innovation in plant breeding that overcomes these limitations. Somatic hybridization is the fusion of two somatic cells from different plant species to produce a hybrid. In this process, the two parent plants are not crossed sexually; instead, their somatic cells are fused in vitro to produce a hybrid plant. Somatic hybridization is a powerful tool in plant breeding because it can be used to combine desirable traits from different plant species that are not sexually compatible. Below we shall explore the major aspects of somatic hybridization, including its history, mechanisms, applications, advantages, and ... Read More

Introduction Protoplast culture is a laboratory technique that involves the isolation of the plant cell from the cell wall. The protoplasts can then be cultured in vitro, allowing for the study of cellular processes, including cell division, differentiation, and regeneration. Protoplast The technique has many applications in plant biotechnology, including the production of new crop varieties, genetic engineering, and the production of plant secondary metabolites. Below we shall look at the importance of protoplast culture in plant regeneration and how it is used to produce new plants. What Are Protoplasts? Protoplasts are cells that have had their cell walls ... Read More

Introduction Handling and sterilization of plant material is an important aspect of any laboratory or plant research facility. Plant material, such as leaves, stems, and seeds, can be contaminated with microbes and other pathogens that can affect the results of research. To ensure the accuracy and reliability of research, it is important to properly handle and sterilize the plant material. In this tutorial, we will discuss various methods of handling and sterilization of plant material. Part 1: Handling of Plant Material Handling of plant material involves the proper collection and preparation of the sample for sterilization. Before collecting plant ... Read More

Introduction Transgenic organisms are living organisms whose genetic material has been artificially modified to introduce specific traits or characteristics. This can be done through genetic engineering, which involves the insertion of specific genes or DNA sequences into an organism's genome. Transgenic organisms have become increasingly important in various fields, including agriculture, medicine, and biotechnology. Genetic and molecular analyses are essential in understanding the mechanisms underlying transgenesis and in evaluating the potential risks and benefits associated with the use of transgenic organisms. Genetic Analysis of Transgenics Genetic analysis involves the study of an organism's genetic material, including its DNA, ... Read More

Embryo Rescue -A Milestone Embryo rescue is a technique that involves the rescue of developing embryos from immature or unpollinated ovules of sexually incompatible plant crosses. It enables the development of hybrid embryos that would otherwise not survive to maturity. Embryo rescue was first introduced in the early 20th century and has since been extensively used in plant breeding, particularly in generating interspecific and intergeneric hybrids. The basis of embryo rescue is the manipulation of the in vitro culture conditions to provide the developing embryo with the necessary nutrients and support for its growth and development. The success of ... Read More