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GATE Section-XL-Q Biochemistry Syllabus
Course Syllabus
Unit: 1
- Organization of life
- Importance of water
- Structure and function of biomolecules −
- Amino acids
- Carbohydrates
- Lipids
- Proteins
- Nucleic acids
- Protein structure, folding and function −
- Myoglobin
- Hemoglobin
- Lysozyme
- Ribonuclease A
- Carboxypeptidase
- Chymotrypsin
Unit: 2
Enzyme kinetics including its regulation and inhibition, Vitamins and Coenzymes
Metabolism and bioenergetics
Generation and utilization of ATP
Metabolic pathways and their regulation −
Glycolysis
TCA cycle
Pentose phosphate pathway
Oxidative phosphorylation
Gluconeogenesis
Glycogen
Fatty acid metabolism
Metabolism of Nitrogen containing compounds −
Nitrogen fixation
Amino acids
Nucleotides
Photosynthesis −
Calvin cycle
Unit: 3
- Biochemical separation techniques −
- Ion exchange
- Size exclusion and affinity chromatography
- Characterization of biomolecules by electrophoresis
- UV-visible and fluorescence spectroscopy and Mass spectrometry
Unit: 4
- Cell structure and organelles
- Biological membranes
- Transport across membranes
- Signal transduction
- Hormones and neurotransmitters
Unit: 5
- DNA replication, transcription and translation
- Biochemical regulation of gene expression
- Recombinant DNA technology and applications −
- PCR, site directed mutagenesis and DNA-microarray
Unit: 6
- Immune system −
- Active and passive immunity
- Complement system
- Antibody structure, function and diversity
- Cells of the immune system −
- T, B and macrophages
- T and B cell activation
- Major histocompatibilty complex
- T cell receptor
- Immunological techniques −
- Immunodiffusion
- Immunoelectrophoresis
- RIA
- ELISA
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