GATE Section-XL-Q Biochemistry Syllabus


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Course Syllabus

Unit: 1

  • Organization of life
  • Importance of water
  • Structure and function of biomolecules −
    • Amino acids
    • Carbohydrates
    • Lipids
    • Proteins
    • Nucleic acids
  • Protein structure, folding and function −
    • Myoglobin
    • Hemoglobin
    • Lysozyme
    • Ribonuclease A
    • Carboxypeptidase
    • Chymotrypsin

Unit: 2

  • Enzyme kinetics including its regulation and inhibition, Vitamins and Coenzymes

  • Metabolism and bioenergetics

  • Generation and utilization of ATP

  • Metabolic pathways and their regulation −

    • Glycolysis

    • TCA cycle

    • Pentose phosphate pathway

    • Oxidative phosphorylation

    • Gluconeogenesis

    • Glycogen

    • Fatty acid metabolism

  • Metabolism of Nitrogen containing compounds −

    • Nitrogen fixation

    • Amino acids

    • Nucleotides

  • Photosynthesis −

    • Calvin cycle

Unit: 3

  • Biochemical separation techniques −
    • Ion exchange
    • Size exclusion and affinity chromatography
    • Characterization of biomolecules by electrophoresis
    • UV-visible and fluorescence spectroscopy and Mass spectrometry

Unit: 4

  • Cell structure and organelles
  • Biological membranes
  • Transport across membranes
  • Signal transduction
  • Hormones and neurotransmitters

Unit: 5

  • DNA replication, transcription and translation
  • Biochemical regulation of gene expression
  • Recombinant DNA technology and applications −
    • PCR, site directed mutagenesis and DNA-microarray

Unit: 6

  • Immune system −
    • Active and passive immunity
    • Complement system
    • Antibody structure, function and diversity
  • Cells of the immune system −
    • T, B and macrophages
    • T and B cell activation
    • Major histocompatibilty complex
    • T cell receptor
  • Immunological techniques −
    • Immunodiffusion
    • Immunoelectrophoresis
    • RIA
    • ELISA

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