The steps involved in observing a slide under a microscope are given below. They are not in proper sequence.I. Focus the object under high power of the microscope.II. Place the slide on the stage of the microscope.III. Arrange the mirror to reflect maximum light to the slide.IV. Focus the object under low power of the microscope.The proper sequence of steps is(a) II, III, IV, I (b) I, II, III, IV(c) IV, III, II, I (d) III, I, II, IV
Correct Answer: (a) II, III, IV, I
Explanation:
- Place the microscopic slide on the stage and fix it with the stage clips.
- Look at the objective lens and stage. Turn focus knob to move stage upward.
- Observe through the eyepiece and move the focus knob till the image comes into focus.
- Arrange the mirror and condenser to focus and reflect the maximum amount of light to the slide.
- With the use of a focus knob, obtain a clear view of the sample and readjust the condenser with a low power objective for the clearest image.
- After a clear image of the sample is obtained with the low power objective, change the objective lens to high power.
- Again, with the use of focus know, obtain a clear view of the sample with high power objective lens.
- Do not let the objective lens touch the slide.
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