List the steps of preparation of temporary mount of a leaf peel to observe stomata.


The steps of preparation of temporary mount of a leaf peel to observe stomata are as follows:-


1. Take a healthy leaf from your garden or the potted plant. 

2. Fold the leaf and remove a part of the peel from the lower surface of the leaf using forceps. 

3. Leave the peel for some time in a watch glass containing water.

4. Add a few drops of safranin stain in the sample through a dropper.

5. After 2-3 minutes, take the peel out and set it on a clean glass slide.

6. Add a drop of glycerin over the peel, and put a clean coverslip gently over it using a needle.

7. Remove the excess glycerin and stain using blotting paper. 

8. Observe the slide first under a low-power, and then under a high-power magnification of a compound microscope.


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Simply Easy Learning

Updated on: 26-Apr-2023

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