A student has to observe a permanent slide of binary fission in Amoeba. Find the correct sequence of steps given below for focusing the object under a microscope.a. Place the slide on the stage, look through the eye-piece and adjust the mirror to get proper illumination.b. Focus the slide sharp using fine adjustment screw.c. Look through the eye-piece and raise the objective lens using coarse adjustment screw till the object is focused.d. Look through the eye-piece and move the slide till the object is visible.(a) a, c, d, b (b) d, c, b, a(c) a, b, d, c (d) a, d, c, b
Correct Answer: (d) a, d, c, b
Explanation:
- Place the microscopic slide on the stage and fix it with the stage clips.
- Look at the objective lens and stage. Turn focus knob to move stage upward.
- Observe through the eyepiece and move the focus knob till the image comes into focus.
- Arrange the mirror and condenser to focus and reflect the maximum amount of light to the slide.
- With the use of a focus knob, obtain a clear view of the sample and readjust the condenser with a low power objective for the clearest image.
- After a clear image of the sample is obtained with the low power objective, change the objective lens to high power.
- Again, with the use of focus know, obtain a clear view of the sample with high power objective lens.
- Do not let the objective lens touch the slide.
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